Anther culture for haploid and doubled haploid production.
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Anther culture is a strategy in which different stages of anthers aseptically taken from unopened flower bud and are cultured in vitro nutrient medium resulting in getting haploid plantlets via either organogenesis or embryogenesis. In microspore culture is also a technique for producing.
Anther culture can result in considerable somaclonal variation, but this has translated into a novel way of inducing new color variants of the spathe, the most marketable part of this ornamental.
A cluster analysis based on anther culture traits grouped 8 cultivars into 2 classes. The effect of growth regulators was not significant for total regeneration ratio, green and albino plants ratio. The results showed that the efficiency of the anther culture method is largely dependent on the plant genotype.
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Anther culture is a technique by which the developing anthers at a precise and critical stage are excised aseptically from unopened flower bud and are cultured on a nutrient medium where the microspores within the cultured anther develop into callus tissue or embryoids that give rise to haploid plantlets either through organogenesis or embryogenesis.
Haploids are plants with a gametophytic chromosome number and doubled haploids are haploids that have undergone chromosome duplication. The production of haploids and doubled haploids (DHs) through gametic embryogenesis allows a single-step development of complete homozygous lines.
Crop Science Abstract -. The yield of haploid production in wheat (Triticum aestivum L.) anther culture has been extremely low with the currently employed methods. The objective of this study was to determine whether variation in anther culture media would affect the yield of haploids.. Paper no. 8802-07, Project no. 1568. Research.
Plant tissue culture involves the culture of all types of plant cells, tissues and organs under aseptic conditions. This definition also extends to the culture of excised embryos and to protoplast culture. An overview of tissue culture techniques and their applications in plant propagation and genetic improvement of plants is presented. The areas under review include: (1) embyro culture, (2.
Anther culture provides an easy to handle in vitro selection for genetic improvisation of characters (Jahne et al., 1991) for superior performance.
Abstract. Regenerants from anther culture of Citrus clementina Hort. ex Tan. cvv. Nules, SRA 63, and Monreal were obtained in different experiments from 1994 to 2002. Genetic analysis of 37 such regenerants was carried out using 4 microsatellite markers that were heterozygous in the parental genotypes. The results showed that in all cases but one the regenerants carried only one or the other.
Anther culture is being used in cereal crop improvement both as a source of haploids and for inducing the new genetic variability. We studied the possibilities of producing the aneuploids in rice by anther culture. Anthers for callus induction were plated on semi solid media. Callus formation was observed 20 days after plating. The anthers became necrotic prior to callus formation.
Research review paper. Chilli peppers — A review on tissue culture and transgenesis.. but the other modes of regeneration as somatic embryogenesis or protoplast-to-plant regeneration and anther culture for androgenic haploid production have all been explored. Table 1.
